Composite

Part:BBa_K772101:Design

Designed by: Mustafa Elitok   Group: iGEM12_Fatih   (2012-09-26)


Self-Destruction Kit: Endolysin + Holin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
    Illegal AgeI site found at 341
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no RBS in the upstream region, so during the assemble procedure, this must be taken care of.


Source

Gene sequences of endolysin and holin are gathered from the Registry.

References